Figure 1.
Chronic stress regulates BDNF expression in CA3 and BLA in contrasting manner.
Data are presented as mean ± SEM. A Experimental design of chronic stress (CIS+1d) followed by micro dissection of area CA3 from transverse hippocampal sections or BLA from coronal sections. B BDNF expression in area CA3 is decreased after chronic stress (Control, n = 5; CIS+1d, n = 5), C Chronic stress up regulates BDNF expression in BLA (Control, n = 6; CIS+1d, n = 8). Chronic stress induced changes in BDNF expression profile is normalized to respective control level. D Serum corticosterone levels are up regulated after chronic stress in comparison to controls (Control, n = 5; CIS+1d, n = 6). Significant differences *, p<0.05; Unpaired, two-tailed, Student's t-test was used to compare stress and control groups.
Figure 2.
Contrasting profiles of BDNF in CA3 and BLA in response to recovery from chronic stress.
Data are presented as mean ± SEM A Experimental design of 21 days of recovery from chronic stress paradigm (CIS+21d). B BDNF expression in area CA3 comes back to control levels post recovery from chronic stress (Control, n = 6; CIS+21d, n = 6), C Chronic stress induced up regulation in BDNF expression in BLA persists even after twenty one days of recovery (Control, n = 4; CIS+21d, n = 6). BDNF expression profile is normalized to respective control level. D The up regulation of serum corticosterone levels persists even after recovery from chronic stress (Control, n = 7; CIS+21d, n = 6). Significant differences *, p<0.05; Unpaired, two-tailed, Student's t-test was used to compare stress and control groups.
Figure 3.
Acute stress modulates BDNF profile differentially in CA3 and BLA.
Data are presented as mean ± SEM. A Experimental design of acute stress (AIS+1d) and recovery for 10 days from acute stress (AIS+10d). B BDNF expression in area CA3 in response to acute stress. Acute stress does not change the BDNF levels in CA3 (Control, n = 9; AIS+1d, n = 9; AIS+10d, n = 6, F(2,21) = 2.11, p>0.05). C Acute stress up regulates BDNF expression in BLA and this up regulation persists even after recovery (Control, n = 6; AIS+1d, n = 5; AIS+10d, n = 6, F(2,14) = 4.82, p<0.05). BDNF expression profile was normalized to respective control levels and evaluated by Fisher's post-hoc. D Effect of acute stress and recovery from acute stress on serum corticosterone levels (Control, n = 11; AIS+1d, n = 5; AIS+10d, n = 6, Kruskal-Wallis ANOVA, χ2(2) = 10.01, p<0.05, Mann-Whitney post-hoc, p<0.05). Significant differences *, p<0.05.
Table 1.
Summary of body weight measures.