Table 1.
Experimental culture conditions used in experiments.
Table 2.
Oligonucleotide primers and hydrolysis probes used in this study.
Table 3.
Linear standard curves for the PC gene and Cm insertion.
Figure 1.
Growth curves of the WT (black circles) and MT (white circles) strains in monoculture experiments under different environmental conditions.
A OLHN (optimal light and high nitrogen concentrations), B OLLN (optimal light and low nitrogen concentrations), C LLHN (low light and high nitrogen concentrations), and D LLLN (low light and low nitrogen concentrations). Error bars represent the standard deviation (N = 3). NO3 was added (dashed line) on day 11 under OLLN conditions, and on day 26 under LLLN conditions.
Table 4.
Cell growth rates (mean values ±SD) of the WT and MT strains estimated under the different culture conditions tested and results of ANOVA/Tukey's test.
Figure 2.
Relationship between cell growth rates and MC production rates of the WT strain.
Each point represents the mean value of triplicate determinations. Rates were calculated between successive sampling points during the exponential growth phase in monoculture experiments (crosses and continuous line), and in co-culture experiments (triangles and dashed line).
Figure 3.
Time-course of the intracellular MC content in monoculture (histograms with black points) and in co-culture (hatched histograms) experiments under different culture conditions.
A OLHN (optimal light and high nitrogen), B OLLN (optimal light and low nitrogen), C LLHN (low light and high nitrogen), and D LLLN (low light and low nitrogen). Error bars represent the standard deviation (N = 3). NO3 was added (dashed line) on day 11 under OLLN conditions, and on day 26 under LLLN conditions.
Figure 4.
Time-course of the relative proportions of the WT (black circles) and MT (white circles) strains in co-culture experiments under different culture conditions.
A OLHN (optimal light and high nitrogen), B OLLN (optimal light and low nitrogen), C LLHN (low light and high nitrogen), and D LLLN (low light and low nitrogen). Error bars represent the standard deviation (N = 3). NO3 was added (dashed line) on day 11 under OLLN conditions, and on day 26 under LLLN conditions.