Figure 1.
Flow chart summarizing the analyses carried out on ESCC cases used from the Golestan Case-Control Study and the number of mutations detected.
Of a total of 160 cases, 119 were fully sequenced for exons (Ex) 2–11 whereas 41 were either not available (NA) or incompletely sequenced. Numbers of samples with mutations (MT) or with wild-type TP53 (WT) are indicated. Among MT cases, the number of cases with 1, 2 or 3 mutation(s) (mut) is given. Below the dotted line, the total number of mutations (120) is broken down into mutations in exons (Ex) 5 to 8 or in other exons.
Table 1.
Number of participants and mutations by demographic, lifestyle, and tumor characteristics.
Figure 2.
Distribution of TP53 mutation types in ESCC.
A: TP53 Mutation types in ESCC from Golestan, exons 2 to 11. Mutations are grouped in categories as defined in the IARC TP53 mutation database (http://www-p53.iarc.fr). B: Comparison of mutation types in exons 5 to 8 of ESCC from Golestan (this study, n = 101) with results from Tehran (n = 85) and Henan Province, China (n = 117) compiled in the IARC TP53 mutation database (http://www-p53.iarc.fr/AdvancedCriteria.asp; search criteria: Esophagus, exons 5 to 8, Squamous Cell Carcinoma, surgery or biopsy, DNA; for China: Linxian and/or Henan, for Iran: Tehran).
Table 2.
Comparison between TP53 mutation patterns in ESCC of patients from Golestan and Tehrana.
Figure 3.
Distribution of TP53 mutation types in ESCC from Golestan according to tea temperature.
Tea temperature was estimated by the time interval between pouring and drinking tea (from 0–1 min to 4 min or more). Mutations are grouped in categories as in Figure 2. *: OR: 6.27, 95% CI, 1.04–37.69 for wild-type TP53 in Group 0–1 min as compared to Group 4 min or more; **: OR: 6.40, 95% CI, 1.16–36.16 for G∶C to A∶T at CpG in Group 0–1 min as compared to Group 4 min or more. Note the absence of G∶C to T∶A, G∶C to C∶G, A∶T to G∶C and A∶T to C∶G mutations in Group 0–1 min.
Table 3.
Associations between patient's characteristics and selected mutation typesa.