Figure 1.
Structures of DBP and analogues.
Figure 2.
Scheme of one possible reaction in permanganate oxidation of polystyrene and its subsequent reaction with DBAP.
Figure 3.
UV absorbance spectra of DBAP, OVA, DBAP-OVA (A) and DBCP, OVA, DBCP-OVA (B).
All the concentrations of the tested substances were 0.5 mg/mL.
Table 1.
Titers and sensitivity of antisera of immunized mice.
Table 2.
Summary of the results of cell fusion and hybridoma selection.
Figure 4.
Effects of pH (A), ionic strength (B) and detergent (C) in assay buffer and blocking reagents (D) on icELISA for DBP.
Each point represents the mean of ±SD of three replicates (three plates on the same day).
Table 3.
Influence of organic solvents in assay buffer on parameters of icELISA.
Table 4.
Absorbance of anti-DBP antibody bound to hapten-coated polystyrene wells.
Figure 5.
Representative icELISA standard curves for DBP.
Each point represents the mean of ±SD of three replicates (one plate on three different days). The standard curve was calculated according to the 4-parameter equation y = (A−D)/[1+(x/C)B)]+D with the following values: for hapten coated icELISA, A = 0.956, B = 0.705, C = 14.6 ng/mL, D = 0.027, R2 = 0.9985; for conjugate coated icELISA, A = 0.892, B = 0.994, C = 106 ng/mL, D = 0.0094, R2 = 0.9976.
Table 5.
Cross-reactivity of some compounds structurally related to DBP by hapten coated icELISA.
Table 6.
Recovery of DBP spiked into water samples.
Figure 6.
Correlation of ELISA and GC-MS analysis of certain household items.
Each point represents the mean of three replicates of one sample.