Figure 1.
HPLC-chromatograms of lyso-GM2 in the brain.
A wild-type mouse (W), an untreated Sandhoff mouse (S), and a Sandhoff mouse treated with the modified Hex B (S+E). Arrows indicate the peak of lyso-GM2.
Figure 2.
MALDI-TOF-MS spectra of lyso-GM2.
Structure of lyso-GM2 (top) and its spectra obtained for the brain of a Sandhoff mouse (bottom).
Figure 3.
Lyso-GM2 levels in the brains and plasma of mice.
Lyso-GM2 levels in brain tissues (A) and plasma (B). Wild-type mice (W,♦), untreated Sandhoff mice (S,▪), and Sandhoff mice treated with the modified Hex B (S+E,▴). Each line indicates the mean±SD. *P<0.05 (t-test).
Figure 4.
GM2 levels in the brains of mice.
GM2 levels in brain tissues of wild-type mice (W,♦), untreated Sandhoff mice (S,▪), and Sandhoff mice treated with the modified Hex B (S+E,▴). Each line indicates the mean±SD. *P<0.05 (t-test).
Table 1.
Plasma lyso-GM2 levels and hexosaminidase activities.