Table 1.
Germination rate of WT and ΔMrCYP52 on 1% alkanes and cuticular hydrocarbons (20 hrs).
Figure 1.
Sequence alignment analysis of MrCYP52 with defined CYP52s.
CYP52A1 (AAA34354), CYP52B1 (CAA78357), CYP52C1 (CAA78358) and CYP52D1 (Q12585) from Candida tropicalis, CYP52E1 (P43083) from Candida apicola. The heme-binding regions and other conserved domains discussed in the text are marked.
Figure 2.
Growth of M. robertsii wild type and ΔMrCYP52 in basal salts containing 1% alkanes.
Figure 3.
RT-PCR analysis of MrCYP52 expression by Wild type M. robertsii transferred from SDB medium to water, SDB, cell free hemolymph (HE), basal medium (BS) containing 1% Manduca larval cuticle (Cut), 1% decane or 1% glucose (MM) for 6 h.
Figure 4.
Induction of WT-PMrCYP52:GFP incubated for one hour.
(A) 1% decane. (B) 1% decane supplemented with 1% glucose. (C) 1% decane supplemented 1% glycerol.
Figure 5.
Time course of induction of WT-PMrCYP52:GFP in BS supplemented with different alkanes.
30 µl 2×107 ml−1 spore suspensions of WT-PMrCYP52:GFP were incubated for 10 h in 0.0125% YE and then transferred to BS supplemented with different carbon source.
Figure 6.
Time course of induction of WT-PMrCYP52:GFP in BS supplemented with decane, octacosane or myristic acid.
40 µl 1% octacosane or myristic acidsolution in hexane was pipette onto glass coverslips and evaporated, leaving a white greasy layer. The coverslips were then placed in polystyrene petri dishes with the alkane layer facing up, and the dishes were inoculated with 30 µl of 1×107 ml−1 spore suspensions in BS medium.
Figure 7.
Induction of WT-PMrCYP52:GFP on locust wings (A) or locust wings treated with hexane(B).