Figure 1.
The seven sampling areas across New Guinea.
The number of species found at each area is given, with percentage of the total species number in parentheses. Pie charts show the number of species found in the area (green) in relation to New Guinea γ diversity (grey). The results rely on the refined dataset (989 individuals and 270 morphospecies). EHL = Eastern Highlands. The frame in Balim outlines the area shown in the detailed map (see Additional Fig. S1).
Figure 2.
a) unsorted sample of edaphic weevils including Trigonopterus and other genera in ethanol. b–c) sorted samples containing each “initial morphospecies” of Trigonopterus. d–e) Dry-mounted specimens of Trigonopterus after DNA extraction and the preparation of genitalia; “refined morphospecies”; examples of four characteristic edaphic species (d) and foliage-frequenting species (e).
Figure 3.
Cox1-based RaxML subtree including three morphospecies for which we sequenced nDNA markers.
Red bar = initial morphospecies hypotheses. Additional bars are species delineation based on nDNA markers (green), GMYC analysis (yellow), clustering at different thresholds using SpeciesIdentifier (blue bars) and the final identification based both on morphological and molecular data (black). Bootstrap values at the nodes are based on 200 replicates. In tip labels, areas where specimens were found are indicated: ARF = Arfak, SOG = Sogeri, EHL = Eastern highlands, BI = Biak, CYC = Cyclops. 1 ARC1109: single female not included in the analyses.
Table 1.
p-distances of 40 specimens, partly representing cryptic species.
Table 2.
Regional and local clustering at a 3% threshold and GMYC of the final dataset.
Figure 4.
Agreement (black) and taxonomic accuracy (red) at thresholds from 1–12%.
Numbers derived from the refined dataset, including 270 morphospecies. Dotted lines define 95% confidence interval of the seven areas. * = Thresholds at which clustering of the complete dataset differs statistically significant from the clustering of the areas.
Table 3.
Summary of the ß-diversity between the four localities of the Balim area.
Table 4.
Summary of the ß-diversity between the six localities of the Eastern Highlands area.