Figure 1.
Cartoon of dynamic wrinkling with a bilayer.
A cartoon showing a uniform lipid bilayer on a flat and stretched PDMS elastomer a. b, real-time surface wrinkling of the elastomer induces curvature dependent molecular and domain redistribution. c, an inset at the bottom depicts nanoscale topography.
Figure 2.
a and b, AFM micrographs of a buckled PDMS substrate, revealing micro- and nano-scale surface corrugations.
Figure 3.
Epifluorescence images of lipid bilayers (1% TR-DHPE, 0.5% GM, 10% CH, 45% DOPC, 43.5% Egg-SM).
(a), a in image of a bilayer on PDMS prior to wrinkling, 10×. (b), an image of the same bilayer in (a) after wrinkling, 20×. (c), an image of a bilayer that was applied to pre-wrinkled PDMS, 10×. Large, oval-shaped domains are visible in (b) and some have been circled in yellow for clarity. Smaller, 1–2 m domains are visible in (c). All scale bars
m. The contrast of panel b has been adjusted. The bright lines seen in (b) and (c) are optical artifacts.
Figure 4.
Transfer of bilayer by micro-contact printing from corrugated PDMS elastomers onto silicon substrates.
(a) Epifluorescence image of PDMS substrate following micro-contact printing. The image reveals a substantial loss of fluorescence from the PDMS hills, which come in contact with the silicon substrate. (b,c) Atomic force microscopy images (2D rendition, Fig. 4 b and 3D rendition, Fig. 4 c). The images indicate transferred height of the lipid phase of nm confirming the presence of single bilayer onto the parent PDMS. Note also that nanoscale features of the hill region (see text) are reproduced in the transfer process.
Figure 5.
Epiflourescence images showing fluorescent cholera toxin bound to the bilayer.
Constrained re-equilibration of a bilayer consisting of TR-DHPE/GM/CH/DOPC/Egg-SM (1/0.5/5/74/19.5) on a dynamically buckled PDMS substrate. Fluorescence images taken after FITC-tagged cholera toxin subunit B was applied to a bilayer on PDMS. a, the Texas Red channel; b, the FITC channel; c, the combined fluorescence channel. Scale bar
m. Panel d compares the linescan of the fluorescence intensities shown in panel c.