Figure 1.
Rose flower development stages.
A. (a) to (f): Morphology of the floral transition in one-time flowering roses (R. wichurana) Schematic representation of the different stages observed during the floral transition in spring is shown in the upper panel from a vegetative meristem (VM) to a floral meristem (FM). a to c: Light microscopy of cross section of meristems. d to f: Environmental scanning electron microscopy images. Black bar: 10 µm. (g) to (k): Rose flower organogenesis stages. Cross sections of floral meristem and young flower buds. Images representing initiation of sepals (stages 1, g), petals (stage 2, i), stamens (stages 3, h) and carpels (stage 4, j). k: hypanthium starts introverting below the floral organs (stages 5). Black bar: 50 µm (g,h,i); 200 µm (j,k). B. Visible rose flower stages. Pictures of rose flowers at flower bud with visible petals (stage VP), open flower stage (OF) and senescing Flower stage (SF).
Figure 2.
Description of the comparisons performed using micrarrays.
A. To identify genes associated with floral initiation in Rosa using R. wichurana (Rw), R. x hybrida cv. Félicité et Perpétue (FP); Comparisons were done in the 2 genotypes; VM1: vegetative meristem stage; VM2: pre-floral meristems; MF: floral meristem. B. Schematic representation showing the rose flower development stages from flower organogenesis (stage 1) to onset of senescing flowers (stage SF). Arrows indicates the different transcriptome comparisons. VP: flower bud with visible petals; OF: open flower; SF: Senescing flower.
Table 1.
Top list of putative floral repressors and activators shared between R. x. wichurana and “Félicité et Perpétue”.
Figure 3.
Real time quantitative RT-PCR (qPCR) analysis of six selected differentially expressed genes during rose floral organogenesis, floral opening and senescence in R. chinensis cv. Old Blush.
qPCR data (black histograms) are compared to the microarray hybridization data (white histograms). Microarray data is presented regardless of Bonferroni test success. Each pair of histograms represent successive comparisons between floral development stages 1+2, 3+4, 5, visible petals (VP), open flower (OF) and senescing flowers (SF).
Table 2.
List of selected floral organogenesis associated genes in R. chinensis cv Old Blush.
Table 3.
List of selected genes associated with early to late flower development in R. chinensis cv Old Blush.
Table 4.
List of selected floral maturation and senescence associated genes in R. chinensis cv Old Blush.
Table 5.
Gossip analysis of GO terms enrichment in late flower development dataset (genes that are differentially expressed at least once during floral maturation and senescence).