Figure 1.
Terminal embryo phenotypes of the strongest known alleles of “true” EMB genes without evidence of gametophyte defects.
(A) Entire dataset of 352 genes. (B) Comparison between unique “true” (UT) EMB genes without a redundant paralog, and the remaining dataset of other (OT) “true” EMB genes that may be redundant (BLASTP match>e-30).
Table 1.
Classification of genes required for embryo and gametophyte development.
Figure 2.
Distribution of protein function classes among different collections of essential genes.
(1) DNA synthesis; repair; (2) RNA synthesis; modification; (3) protein synthesis; (4) Protein modification; transport; (5) protein degradation; (6) chromosome dynamics; (7) transcriptional regulation; (8) signaling pathways; (9) energy; electron transport; (10) metabolism; (11) cell structure; membrane function; trafficking. Significant differences are observed for function classes 2 (χ2 = 6.22; p<0.05), 3 (χ2 = 6.77; p<0.05), and 11 (χ2 = 16.7; p<0.001). Excluded are genes with functions that remain to be classified (15 “true” EMB; 5 EMG/GEM; 3 GAM) and those with uncertain or unknown functions (57 “true” EMB; 3 EMG/GEM; 6 GAM).
Table 2.
Unique “true” EMB genes with a documented preglobular embryo phenotype.
Table 3.
Summary of pollen development transcriptome data for selected essential genesa.
Table 4.
Summary of microsporocyte transcriptome data for selected essential genesa.