Figure 1.
Electrophoretic analysis of restriction mapping.
Lane 1 shows the pCR®2.1-TOPO® vector (3923 bp) above, which is cleaved by EcoRI (Invitrogen, Karlsruhe) and below the amplified 18S rRNA gene fragment (1724 bp) with the correct orientation (lane 2 with the incorrect orientation). The controls show the amplified PCR product (lane 3) and vector without insert (lane 4). M: marker λ-EcoRI +HindIII-Marker-Mix 3 (Fermentas, St. Leon-Rot) 1 µg.
Table 1.
Newly collected acanthocephalans.
Table 2.
Acanthocephala and Rotifera specimen information and GenBank accession numbers.
Table 3.
Tree statistics for rDNA data set.
Figure 2.
Bayesian consensus phylogram for Acanthocephala relationship based on the SSU rDNA data set.
Rotifera is used as outgroup, acanthocephalans are classified as indicated on the right site of the graphic. This tree illustrates the hypothesis that the order Echinorhynchida (blue) and Polymorphida (red) have a paraphyletic arrangement. The branch length scale is the number of substitutions per site.
Figure 3.
SEM (scanning electron microscope) micrographs of Palaeacanthocephala.
(A) Proboscis of male Rhadinorhynchus pristis from Gempylus serpens (Indonesia, Indian Ocean) armed with regular hooks a and basal hook annulus. (B) Praesoma of female R. lintoni from Selar crumenophthalmus (Hawaii, Pacific) with irregular arrangement of trunk hooks. (C) Praesoma of Gorgorhynchoides golvani from Platycephalus arenarius (Indonesia, Indian Ocean) regular arrangement of surface hooks. (D) Habitus of Serrasentis sagittifer from Platycephalus arenarius (Indonesia, Indian Ocean) with hooks are transformed into strong plates arranged as combs. (E) Habitus of Pomphorhynchus laevis from Platichthys flesus (Baltic Sea) shows any trunk hooks on bulb, neck and trunk. (F) Praesoma of Bolbosoma vasculosum from Lepturacanthus savala (Indonesia, Indian Ocean) formed in the shape of a bulb, and armed with regular hooks which are arranged in two rings. Scale bars: A 400 µm, B, D, F, 200 µm, E 100 µm.