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Table 1.

The 24 most represented transcripts in the A. ostenfeldii EST library.

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Figure 1.

KOG category distribution of unique sequences of the A. ostenfeldii cDNA library.

Numbers of unique sequences and their share of total number are shown. The category “poorly characterized” includes “general function prediction only (R)” and “function unknown (S)”.

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Figure 2.

Alignment of ESTs of Alexandrium spp. showing spliced leader (SL) and relict SL-repeats with different conservation levels.

A reference SL consensus sequence (first sequence in lower case) is shown. The generated consensus sequence, sequence conservation and sequence logo are represented under the alignment. See File S1 for the alignment of the complete SL-containing EST set.

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Table 2.

Distribution of stop codons in A. ostenfeldii and A. minutum.

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Figure 3.

Distribution of features of A. ostenfeldii genomic sequences illustrated with combined BAC and fosmid data.

“Rest” includes genes (16), viral sequence, SINE, LINE, LTR retrotransposons, DNA transposons, RNA pseudogenes (snRNA, scRNA, rRNA), and satellites. Repetitive sequences were identified by contig generation within each library and BLASTn between the two libraries. Transposons were identified as described in the text. The remaining features were determined by RepeatMasker analysis. The category “Without distinguishing features” includes all single reads with no BLAST or RepeatMasker hit.

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Figure 4.

Large tandem arrays within the five most repetitive fosmid contigs.

Contigs comprise 98 reads (aostB4-F-a-05f01.r1), 338 reads (aostB5-F-a-20a11.r1 and one BAC read), 277 reads (aostB5-F-a-09f07.r1), 71 reads (aostB5-G-a-22e01.f1) and 313 reads (aostB5-F-a-17b10.f1).

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Table 3.

Distribution of features of A. ostenfeldii genomic sequences.

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Table 4.

Repeats 1–4 that form large tandem arrays within the five most repetitive fosmid contigs.

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Table 5.

RepeatMasker identification of putative transposon sequence stretches of the A. ostenfeldii genomic sequence.

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