Table 1.
Wheat microsatellite (SSR) markers used in the present study, their assignment to peaks I–IV on flow karyotypes of Ae. umbellulata, Ae. comosa, Ae. biuncialis and Ae. geniculata and their positions on Aegilops chromosomes.
Figure 1.
Flow cytometric chromosome analysis.
Histograms of relative fluorescence intensity (flow karyotypes) were obtained after the analysis of DAPI-stained chromosome suspensions prepared from Ae. umbellulata MvGB470 (2n = 2x = 14, UU) (A); and Ae. comosa MvGB1039 (2n = 2x = 14, MM) (B); and from their natural hybrids Ae. biuncialis MvGB382 (2n = 4x = 28, UbUbMbMb) (C); and Ae. geniculata AE1311/00 (2n = 4x = 28, UgUgMgMg) (D).
Figure 2.
Distribution of DNA repeats on mitotic chromosomes.
Fluorescence in situ hybridization (FISH) was done with probes for repetitive DNA: pSc119.2 (green), Afa family (red) and pTa71 (yellow) on mitotic metaphase spreads of Ae. umbellulata MvGB470 (A); Ae. comosa MvGB1039 (B); Ae. biuncialis MvGB382 (C); and Ae. geniculata AE1311/00 (D). Scale bars = 10 µm.
Figure 3.
Mitotic metaphase chromosomes were sorted from individual peaks of flow karyotypes of Ae. umbellulata, Ae. comosa, Ae. biuncialis and Ae. geniculata. Sorted chromosomes were identified after FISH with probes for repetitive DNA: pSc119.2 (green), Afa family (red) and pTa71 (yellow) (Inverse labelling of probes is marked by asterisk). The chromosomes are shown in peaks where their frequencies were the highest. For each chromosome, four to five representative examples are given.
Table 2.
Assignment of chromosomes to peaks of flow karyotypes of Ae. umbellulata, Ae. comosa and their natural tetraploid hybrids Ae. biuncialis and Ae. geniculata.
Figure 4.
Physical mapping of microsatellite (SSR) markers.
PCR products of wheat SSR markers Xcfa2040 (A); and Xcfa2134 (B–C) amplified from wheat cv. Chinese Spring, Ae. umbellulata MvGB470, Ae. comosa MvGB1039, Ae. biuncialis MvGB382 and Ae. geniculata AE1311/00 and from chromosomes of particular peaks of flow karyotypes (I, II, III, IV) (A–B); and from wheat-Ae. geniculata TA2899 addition lines (C). Black arrows, white arrows, black arrowheads and white arrowheads indicate bands specific for Ae. umbellulata, Ae. comosa, Ae. biuncialis and Ae. geniculata, respectively.