Figure 1.
Representative photomicrographs of Thionin-stained sections from anterior, medial, and posterior EWcp (numbers indicate distance from bregma) taken from mice that underwent (A) successful EWcp lesion surgery or (B) sham surgery. Lesions generally ablated large portions of the anterior and medial EWcp, leaving minimal damage to surrounding tissue. Sham animals displayed no evidence of damage to the EWcp, despite occasional visibility of the electrode tract (posterior panel). White arrows point toward intact EWcp observed in sham animals.
Figure 2.
Effects of EWcp lesion on 2-BC EtOH drinking in Ucn1 KO and WT mice.
(A) EtOH Intake (g/kg), (B) EtOH Preference, and (C) Total Fluid Consumption (mL/kg) of male Ucn1 KO and WT mice following either sham surgery or EWcp lesion. Asterisks indicate significant difference from the respective WT-Sham group (*p≤.005, **p≤.001, ***p<.0001). Numbers in parentheses indicate group sizes. The same animals contributed to panels A, B, and C.
Figure 3.
Involvement of Ucn1 and CRF-R2 in EtOH-induced reward and aversion.
Graphs show percent change in time spent on Grid Floor between the Pre-Test and the Test following (A) EtOH-CPP in Ucn1 KO and WT mice, (B) EtOH-CPP in CRF-R2 KO and WT mice, and (C) EtOH-CPA in Ucn1 KO and WT mice. Multiple asterisks indicate significant difference between WT subgroups (**p<.001, ***p<.0005). Single asterisk indicates significant main effect of subgroup (*p<.05). Numbers in bars indicate group sizes.