Table 1.
Summary of Human adenovirus (HAdV) detection by real-time PCR using the LightCycler 2.0 and J.B.A.I.D.S. in cultured isolates from the CDPH.
Figure 1.
Standard curves for amplification of HAdV-C1, -C2, -C5, and -C6.
Detection by real-time LightCycler PCR in a series of dilutions of genomic HAdV-C1, -C2, -C5, and -C6 DNA. Linear regression of the standard curves for (A) HAdV- C1, (B) HAdV-C2, (C) HAdV-C5, and (D) HAdV-C6. We defined the LLOD as the last dilution before the crossing points ceased to increase.
Table 2.
LLOD for HAdV singleplex reactions (genome copies/reaction).
Table 3.
Specificity table of each HAdV assay.
Figure 2.
Primer design for type specific primer/probe pairs in species HAdV-C hexon.
Table 4.
Primers for real-time PCR amplification of adenovirus. The accession numbers for the viral genome sequences are HAdV-C1 (AF534906), HAdV-C2 (AC_000007), HAdV-C5 (AC_000008), HAdV-C6 (FJ349096).