Figure 1.
Expression of Wnt Ligands at Stage 25.
Whole mount in situ hybridization of Xenopus embryos at stage 25 for Wnt1 (A), Wnt2b (B), Wnt3a (C), Wnt4 (D), Wnt5a (E), Wnt5b (F), Wnt6 (G), Wnt7a (H), Wnt7b (I), Wnt7c (J), Wnt8a (K), Wnt8b (L), Wnt9a (M), Wnt9b (N), Wnt10a (O), Wnt10b (P), Wnt11 (Q), Wnt11b (R) and Wnt16 (S). Inset in D shows close-up of the pronephric anlage. All images are of the same magnification and the scale bar corresponds to 0.5 mm.
Figure 2.
Expression of Wnt Ligands at Stage 35.
Whole mount in situ hybridization of Xenopus embryos at stage 35 for Wnt1 (A), Wnt2b (B), Wnt3a (C), Wnt4 (D), Wnt5a (E), Wnt5b (F), Wnt6 (G), Wnt7a (H), Wnt7b (I), Wnt7c (J), Wnt8a (K), Wnt8b (L), Wnt9a (M), Wnt9b (N), Wnt10a (O), Wnt10b (P), Wnt11 (Q), Wnt11b (R) and Wnt16 (S). Insets in D and M show close-ups of the pronephric tubular region. All images are of the same magnification and the scale bar corresponds to 0.5 mm.
Figure 3.
Expression of Wnt Receptors at Stage 25.
Whole mount in situ hybridization of Xenopus embryos at stage 25 for Fzd1 (A), Fzd2 (B), Fzd3 (C), Fzd4 (D), Fzd5 (E), Fzd6 (F), Fzd7 (G), Fzd8 (H), Fzd9 (I), Fzd10 (J), Ror2 (K) and Ryk (L). All images are of the same magnification and the scale bar corresponds to 0.5 mm.
Figure 4.
Expression of Wnt Receptors at Stage 35.
Whole mount in situ hybridization of Xenopus embryos at stage 35 for Fzd1 (A), Fzd2 (B), Fzd3 (C), Fzd4 (D), Fzd5 (E), Fzd6 (F), Fzd7 (G), Fzd8 (H), Fzd9 (I), Fzd10 (J), Ror2 (K) and Ryk (L). Inset in A, F and H show close-ups of the pronephric tubular region. All images are of the same magnification and the scale bar corresponds to 0.5 mm.
Figure 5.
Expression of Wnt Signaling Intermediates at Stage 25.
Whole mount in situ hybridization of Xenopus embryos at stage 25 for Dvl1 (A), Dvl2 (B), Dvl3 (C), Prickle1 (D), Prickle2 (E), Prickle3 (F), Celsr1 (G), Celsr2 (H), Celsr3 (I), Vangl1 (J) and Vangl2 (K). All images are of the same magnification and the scale bar corresponds to 0.5 mm.
Figure 6.
Expression of Wnt Signaling Intermediates at Stage 35.
Whole mount in situ hybridization of Xenopus embryos at stage 35 for Dvl1 (A), Dvl2 (B), Dvl3 (C), Prickle1 (D), Prickle2 (E), Prickle3 (F), Celsr1 (G), Celsr2 (H), Celsr3 (I), Vangl1 (J) and Vangl2 (K). Inset in G shows close-up of the pronephric tubular region. All images are of the same magnification and the scale bar corresponds to 0.5 mm.
Figure 7.
Pronephros Expression of Wnt Signaling Components.
Paraplast sections of Xenopus embryos processed for whole mount in situ hybridization with Wnt4 (A,A’), Wnt5b (B,B’), Wnt8a (C,C’), Wnt9a (D,D’), Wnt11 (E,E’), Ryk (F,F’), Fzd1 (G,G’), Fzd2 (H,H’), Fzd4 (I,I’), Fzd6 (J,J’), Fzd7 (K,K’), Fzd8 (L,L’), Dvl1 (M,M’), Dvl2 (N,N’), Prickle1 (O,O’), Celsr1 (P,P’), Vangl1 (Q,Q’) and Vangl2 (R,R’) at stage 25 and 35. Sections are through the tubular area of the pronephros. Closed arrowheads indicate distal tubular segments; open arrowheads point towards individual dvl1-positive cells. All panels depicting embryos at stage 25 and 35, respectively are of the same magnification; a representative scale bar corresponding to 0.1 mm is shown in panels R,R’.
Figure 8.
Summary of Different Wnt Signaling Expression Patterns.
Schematics of stage 25 and 35 cross sections summarizing the in situ hybridization data; genes following the same expression pattern are grouped together and their respective expression domains are indicated in red (or light red in the case of weak expression). dt; distal tubules; g, glomus; pa, pronephric anlage; pt, proximal tubules; ns, nephrostomes.