Figure 1.
Growth curve of Burkholderia cepacia and isocitrate dehydrogenase activity at the different phases of growth.
The culture was inoculated in LB medium and grown at 37°C from 0 h to 24 h. It was harvested at every 2 h intervals and the bacterial density was determined at OD600 nm. An isocitrate dehydrogenase assay to specifically detect bacterial lysis in the culture supernatant was preformed. These experiments were conducted in three independent replicates.
Table 1.
Mean percentage of invasion (%) with standard deviation MOI 1∶10 and1∶50.
Figure 2.
The intracellular survival assay of B. cepacia (mid-logarithmic and early-stationary phases of growth) after 3 hours of post-infection with MOI of 1∶10 and 1∶50 was as Log10 cfu/ml of the bacteria recovered.
These experiments were conducted in three independent replicates. The error bars indicate the standard deviation. The significance has been indicated using *.
Figure 3.
Effect of Burkholderia cepacia culture supernatant on A549, human lung epithelial cells.
The cytotoxicity effect was determined using MTT assay. The experiment was performed in triplicates with RPMI and LB media used as control. The error bars indicate the standard deviation. The error bars indicate the standard deviation.
Figure 4.
Analysis of B. cepacia culture supernatant by 2-DE.
The supernatant of B. cepacia grown to mid-logarithmic (panel A) and early-stationary (panel B) phases in LB medium were prepared using TCA precipitation method and analysed using 2-DE. A total of 400 µg of culture supernatant was separated on an IPG strip pH 4–7 in the first dimension, followed by the separation on SDS-12% PAGE for the second-dimension separation. The separated proteins were detected by CBB G-250 staining. Marked spots indicate proteins that were identified. N refers to spots that were not possibly identified by MALDI-TOF.