Table 1.
Strains and plasmids used in this study.
Table 2.
Primers used in this study.
Figure 1.
Examination of com mutants by competitive index analysis in a rabbit model of endocarditis.
Each symbol represents data from a single rabbit. (A) CI values from rabbits inoculated with 108 CFU. Median CI values from the combined results of two experiments are indicated in parentheses and by horizontal bars. The CI value for each rabbit was calculated as the ratio of mutant/JFP36 in the vegetation homogenate divided by the mutant/JFP36 ratio in the inoculum. Neither median value was significantly different from 1.0. (B) Recovery of competing strains from infected vegetations at multiple inoculum levels. Each pair of connected circles indicates recovery of JFP36 (filled circles) and JFP49 (open circles) from the same rabbit. Data for the 108 inoculum are from experiments depicted in panel A. Dashed line, limit of detection.
Table 3.
Recovery of S. sanguinis strains from experimentally infected rabbits.
Figure 2.
Assessment of IE pathology in rabbits inoculated with JFP36 or com mutants.
Rabbits were inoculated with 108 CFU of JFP36, JFP49 (comCDE), or JFP45 (comX). Data were combined from two separate experiments, each of which included all three strains. (A) Survival curve. (B) Total weight loss 5 days post-infection. Horizontal dashed line represents no weight loss. (C) Mass of aortic valve vegetations 5 days post-infection. For B and C, each symbol represents the value from a single rabbit, and horizontal lines indicated median values. No significant differences among strains were observed in any of the analyses.