Figure 1.
Differentially expressed genes overlapping in multiple time points in old versus young injured rat iliac arteries.
A. Venn diagram shows the number of differentially expressed genes determined by our analysis of 3 different time points in old versus young injured rat iliac arteries. Gene lists were compared to find common differentially expressed transcripts. A 2.0 fold change and p<.05 significance cut-offs were used. No multiple correction was employed. B. Heat map of Myl9 gene expression levels in multiple time points in old versus young injured rat iliac arteries. Myosin Light chain 9, Myl9, was significantly downregulated at 30 mins (−2.2 fold), highly upregulated at 3 days (4.6 fold), and upregulated at 7 days (2.9 fold). Color bar shown in Log2.
Figure 2.
Differentially expressed genes overlapping in different age groups of 30 min-injured rat iliac arteries.
A. Venn diagram shows lists of differentially expressed genes determined by our analysis of 3 different pairs of age groups of 30 min-injured rat iliac arteries. Gene lists were compared to find common differentially expressed transcripts. A 2.0 fold change and p<.05 significance cut-offs were used. No multiple correction was employed. B. Heat map of differentially expressed genes overlapping in different age groups of 30 min-injured rat iliac arteries.12 genes overlapped in old versus young, old versus old, and young versus young, 30 min-injured rat iliac arteries. Color bar shown in Log2.
Figure 3.
Differentially expressed genes overlapping in healthy aging rat iliac arteries and aortas.
A. Venn diagram shows differentially expressed genes determined by our analysis of healthy aging rat iliac arteries and healthy aging rat thoracic aortas. The two gene lists were compared to find common differentially expressed transcripts. A 2.0 fold change and p<.05 significance cut-offs were used. No multiple correction was employed. B. Heat map of differentially expressed genes overlapping in healthy aging rat iliac arteries and aortas. Selected genes, including Myl9, from the 114 transcripts overlapping in healthy aging iliac arteries and thoracic aortas, are shown in a heatmap displaying their expression levels in young and old aortas. Color bar shown in Log2.
Figure 4.
Myl9 immunostaining of old (22 months) and young (3 months) non-injured rat iliac arteries.
Shown are representative images of young and old healthy iliac arteries immunostained for Myl9. Results confirm over-expression of Myl9 in the older group and point to concentration of Myl9 expression in the endothelial layer of the iliac arteries.
Figure 5.
Myl9 immunostaining of old (22 months) and young (3 months) injured rat iliac arteries.
Shown are representative images of young and old iliac arteries 3 days post injury, immunostained for Myl9. Results confirm over-expression of Myl9 in the older injured group and point to concentration of Myl9 expression in the smooth muscle layer of the iliac arteries during this post injury transition period.
Figure 6.
Myl9 immunostatining of old (22 months) and young (3 months) injured rat iliac arteries.
Shown are representative images of young and old iliac arteries 30 days post injury, immunostained for Myl9. Results confirm over-expression of Myl9 in the older injured group and point to concentration of Myl9 expression in the endothelial layer of the iliac arteries during this post injury period.