Figure 1.
Chemical structure of erythrosine B (ER).
Figure 2.
Aβ monomers were incubated for one to three days in the absence (no ER) (far-left panels) and the presence of 1x (middle-left panels), 5x (middle-right panels), or 10x ER (far-right panels) and visualized by TEM. Scale bars are 100 nm.
Table 1.
Length distribution of Aβ aggregates incubated in the presence of 1x, 5x, and 10x for one, two, and three days.a
Figure 3.
ThT fluorescence of Aβ samples.
Aβ monomers were incubated for four days in the absence (no ER) or in the presence of 0.01x, 0.1x, 0.5x, 1x, 3x, 5x, or 10x of ER (A). Preformed amyloid fibrils (72 hrs) were mixed with varying concentrations of ER (1x, 3x, and 10x ER). ThT fluorescence was measured in arbitrary units (a.u.). Values represent means ± standard deviation (n = 3).
Figure 4.
Dot-blotting of Aβ samples using four Aβ-specific antibodies.
Aβ monomers were incubated at 37°C in the absence (no ER) or presence of the indicated concentrations of ER (from 1x to 10x) for up to 3 days. Samples were spotted onto a nitrocellulose membrane and immunostained with the 4G8, 6E10, A11, or OC antibody.
Figure 5.
TEM images of Aβ aggregates after three day incubation.
Aβ monomers were incubated for three days in the absence (no ER) or in the presence of 1x, 5x, or 10x ER and visualized by TEM. Scale bars are 20 nm.
Figure 6.
Viability of neuroblastoma SH-SY5Y cells treated with Aβ samples (5 µM) formed in the absence or presence of 10x ER incubated at 37°C for one to three days, measured by MTT reduction.
Values represent means ± standard deviation (n≥3). Values are normalized to the viability of cells administered with PBS only. Two-sided Student's t-tests were applied to the data (* P<0.001; ** P<0.005).
Figure 7.
Viability of neuroblastoma SH-SY5Y cells treated with Aβ samples (5 µM) incubated at 37°C for one day in the absence of (no ER) or in the presence of 1x, 3x, 5x, or 10x ER, measured by MTT reduction.
Values represent means ± standard deviation (n≥3). Values are normalized to the viability of cells administered with PBS only. Two-sided Student's t-tests were applied to the data (* P<0.001; NS: not significant).