Figure 1.
MEGAN comparison of the bacterial taxonomic profiles of genomic DNA (red) and cDNA-derived sequences (blue) from tick sample Ir 1-4.
The height of the bars corresponds to the number of hits for each genus. ‘Not assigned’ indicates sequences matching to sequences in the NCBI database that are not assigned to taxa. ‘No hits’ indicates reads for which no sequence match was found in the BLASTX analysis.
Table 1.
454 GS FLX and Illumina GAIIx sequencing statistics of three single unfed adult I. ricinus ticks and number of sequence reads matching to reference genomes.
Figure 2.
inGAP mapping of genomic DNA-derived sequences obtained from single I. ricinus ticks (Illumina paired-end reads) to the reference chromosomes of pathogenic Borrelia species.
For the purpose of presentation the linear chromosomes of Borrelia spp. are circularized. The outer circle designed as ‘READS’ shows the coverage plot over the reference chromosome, while the inner circle displays the amount of estimated nucleotide mismatches. (A). Genomic DNA-derived sequences from Ir 13-4 mapped to the B. garinii PBi chromosome (NC_006156). (B) Genomic DNA-derived sequences from Ir 13-4 mapped to the four contigs of the B. garinii PBr chromosome (WGS ABJV00000000). (C) Genomic DNA-derived sequences from Ir 20-8 mapped to the B. afzelii PKo chromosome (NC_008277).
Figure 3.
MEGAN comparison of bacterial taxonomic profiles of four tick pools (reflecting different life stages and geographic provenience) based on the V6 amplicon 16S rRNA gene sequence reads analyzed against the Ribosomal Database.
The height of the bars corresponds to the number of hits for each genus. Highlighted in light yellow are bacteria genera recognized as tick-borne pathogens or tick endosymbionts.
Figure 4.
Canonical correspondence analysis ordination diagram.
Ordination of sites along the first two canonical axes and the environmental variables, represented by arrows are showed. AD: tick stage, TN: geographic region. Dots represent sites, i.e. 1: nymph ticks, TN region; 2: nymph ticks, BL region; 3: adult ticks, TN region; 4: adult ticks, BL region. Bacterial taxa were omitted for clarity.
Table 2.
Taxa occurrence breadth in bacterial community of ticks (Levins index, Bsta) and taxa occurrence overlap (Pianka index, O) between tick stages, and geographic regions. Indexes vary between 0 and 1.
Table 3.
Summary of Canonical Correspondence Analysis: Eigenvalues of first two ordination axes, site and biplot scores.