Figure 1.
Effect of vorinostat on IGF-I-mediated signal transduction.
Ishikawa and USPC-2 cells were treated for 24 h with vorinostat (or left untreated) and/or IGF-I during the last 10 min of the incubation period. Whole-cell lysates (100 µg) were resolved on SDS-PAGE and immunoblotted with antibodies against pIGF-IR, TIGF-IR, pAKT, TAKT, pERK1/2, TERK1/2, p21, and actin. The figure shows the result of a typical experiment, repeated three times with similar results. IGF-IR phosphorylation in USPC-2 cells was significantly lower than in Ishikawa cells and was detected by Western blot analysis only after longer exposure times of blots to X-ray film. Therefore, this experiment does not allow comparison between both cell lines in terms of levels of expression or phosphorylation.
Figure 2.
Effect of vorinostat on apoptosis and acetyl-histone H3.
Western blot analysis of PARP (A) on Ishikawa and USPC-2 cells and caspase 3 (B), Bcl2 (C) and caspase 8 (D) on USPC-2 cells. Cells were treated with vorinostat for 24 h in the absence or presence of IGF-I. After incubation, cell lysates were prepared and electrophoresed (100 µg for Bcl2 or 70 µg for caspases 3 and 8). Caspase 3 expression in USPC-2 cells was detected after a long exposure time of blots to X-ray film. Results are representative of three independent experiments.
Figure 3.
Effect of vorinostat on p53 and pTEN expression.
Ishikawa and USPC-2 cells were treated for 24 h with vorinostat (or left untreated) and/or IGF-I. Whole-cell lysates (100 µg) were resolved on SDS-PAGE and immunoblotted with antibodies against p53, pTEN, acetyl-histone H3 and actin. Western blot analysis revealed that basal p53 levels were much lower in USPC-2 than in Ishikawa cells and were detected after long exposure times. Results represent a typical experiment out of three independent experiments.
Figure 4.
Regulation of IGF-IR promoter activity by vorinostat in endometrial cancer cells.
Ishikawa and USPC-2 cells were transiently transfected with an IGF-IR promoter-luciferase reporter plasmid, p(−476/+640)LUC, which contains most of the proximal region of the IGF-IR promoter, and a ß-galactosidase vector. Promoter activity is expressed as luciferase values normalized for ß-galactosidase. A value of 100% was given to the promoter activity in the absence of vorinostat. Results are mean ± S.E.M. (three independent experiments); p*<0.01 versus untreated cells.
Figure 5.
Effect of vorinostat on endometrial cancer cells proliferation.
Cells were plated in 24-well plates at a density of 2×104 cells/well for Ishikawa (A) and 3×104 cells/well for USPC-2 (B). The bars represent the mean ± S.E.M. of three independent experiments, performed each in triplicate; * p<0.05 versus untreated cells.
Table 1.
Effect of vorinostat on the cell cycle in endometrial cancer cells.
Figure 6.
Effect of vorinostat on the mTor and Ampk signaling pathway in endometrial cancer cells.
Ishikawa and USPC-2 cells were treated with vorinostat and/or IGF-I, and cell lysates (100 µg) were prepared after 24 h. Western blot analysis was performed with antibodies against pmTor, T-mTor, p-Ampk, T-Ampk, p21, cyclin D1, PI3K and actin. The figure shows the results of a typical experiment, repeated three times with similar results.
Figure 7.
Colony formation in soft agar.
A total of 1.5×104 Ishikawa cells and 1.2×104 USPC-1 cells were seeded on 6-well plates in soft agar. The formed colonies were photographed at a magnification of 40× and 200× (A) and colonies with diameters greater than 3 mm were counted (B) at day 22. The bars represent the mean ± S.E.M. of three independent wells; * p<0.05 versus untreated cells.
Figure 8.
Effect of vorinostat on wound healing assays in endometrial cancer cells.
Wounds were made on monolayers of Ishikawa (A) and USPC-2 (B) cells grown to 100% confluence. Untreated (control) cells or cells treated with vorinostat and/or IGF-I were photographed just after scratch (time 0), and after 48 h and 72 h. Results presented here are representative of triplicate independent samples of each cell line.
Figure 9.
Effect of vorinostat and/or antibody MK-0646 on apoptosis and IGF-IR protein levels.
Ishikawa and USPC-2 cells were treated with vorinostat for 24 h, separately and in combination with MK-0646, in the presence or absence of IGF-I. Whole-cell lysates (70 µg for caspase 9 and 100 µg for total IGF-IR and p21 proteins detection) were resolved by SDS-PAGE and immunoblotted with antibodies against T-IGF-IR and p21 (A) and caspase 9 (B). The figure shows the results of a typical experiment, repeated twice with similar results.