Table 1.
Gene expression ratios among different groups and their comparisons in PPD-stimulated PBMCs by microarray analysis with P value <0.05.
Table 2.
Significantly regulated genes in PPD-stimulated PBMCs in pair-wise comparisons with ratio >4.
Figure 1.
Venn diagram of differentially expressed genes in PBMCs samples following PPD stimulation with P value <0.05 by Student's t-test and fold change >2.0 from: A, Pair-wise comparisons between three study groups (LTBI vs. HC; TB vs. HC; TB vs. LTBI); B, Transcription profiles of tuberculosis infection and active disease (TB<BI group vs. HC group; TB group vs. LTBI&HC group).
The total numbers of genes in panel A and B were 506 and 304 respectively.
Figure 2.
Unsupervised hierarchical cluster analysis of 506 differentially expressed genes in the pair-wise comparisons.
There are 4 samples in each group. Pseudocolors indicate differential expression (red, up-regulation; green, down-regulation; black, no change in expression).
Table 3.
Significantly regulated genes in PPD-stimulated PBMCs unique for tuberculosis infection and active disease with ratio >4.
Figure 3.
Scatter plots of four discriminatively expressed genes between TB and LTBI by qPCR with P value <0.01.
Horizontal bar, median fold change of each group following PPD stimulation. ** significant difference: 0.001< P value <0.01.
Figure 4.
The combination of CXCL10, ATP10A and TLR6 provides the best discrimination between TB patients and LTBI individuals.
The sensitivity and specificity of this three-gene panel was 80% and 89% respectively. 85% individuals were correctly classified. TB group, n = 25; LTBI group, n = 36. Rectangle: internal nodes; Oval and hexagon: terminal nodes showing the number finally determined as TB and LTBI, respectively.
Table 4.
ROC analysis of selected genes for discrimination between TB group and LTBI group.
Table 5.
demographic characteristics of the study populations.