Figure 1.
Gene expression profiles of HAB vs. LAB mice in multiple brain regions.
(A) gene expression confirmed by quantitative PCR and (B) as detected by microarray-based gene expression analysis. N = 6–10 per group. Data are presented as means and for (A) +SEM; T p<0.1, * p<0.05, ** p<0.01, *** p<0.001.
Figure 2.
Region-specific gene expression in HAB vs. LAB mice.
Expression (A) of Syntaxin3 (Stx3) in the basolateral amygdala (BLA) and (B) of synaptotagmin 4 (Syt4) in the BLA, the central amygdala (CeA) and the hypothalamic paraventricular nucleus (PVN) as measured by quantitative PCR and (C) Syt4 quantified from in situ hybridization. Data are presented as means +SEM; T p<0.1, * p<0.05, ** p<0.01.
Figure 3.
Cathepsin B (Ctsb) gene sequence of HAB vs. LAB mice.
Polymorphic sites are shown with positions in the coding sequence referenced to the transcription start in the spliced mRNA in bp. Exons and untranslated regions (UTRs) are indicated by boxes (exons shaded, UTRs completely filled black or white).
Figure 4.
Phenotypes of Cathepsin B (Ctsb) knockout (KO) vs. heterozygous (HET) and wildtype (WT) mice.
(A) Locomotion of male and (B) female mice in the open field, (C) anxiety-related behavior of male and (D) female mice as measured in the open field; (E) depression-like behavior of male and (F) female mice as reflected by the forced swim test. Data are presented as means +SEM; * p<0.05.