Table 1.
Viruses and plasmas used in antibody neutralization assays.
Figure 1.
Neutralizing antibody titers of plasmas tested with autologous and heterologous viral envelopes.
Plasma samples are from chronically infected individuals not currently on treatment. Titers are expressed as 1/plasma dilution. Higher numbers indicate greater inhibition (titer). Green color indicates positive neutralization activity which is significantly greater than inhibition of the specificity control, aMLV. Dashed red line indicates autologous plasma/virus pair. na = not available.
Figure 2.
Cellular immune responses induced by the DNA vaccine candidates.
Intra-cellular INF gamma (IFNγ) staining IFNγ production was measured by FACS analysis after intracellular staining of IFNγ). Balb/C mice (n = 6 per group) were inoculated IM with (i) pORT ConcTat NG, (ii) pORT Gag 203 , (iii) pORT Env NG2 , (iv) pORT Env NG9 , (v) pORT gp120 C N54 (positive control), (vi) pORT1a clade (negative control). One week after the second/third booster immunization, spleen cells were isolated and tested for specific cellular immune responses by measuring IFN-g production after stimulation with pools of overlapping 15-mer peptides. Shown is the total number of CD8+ IFNγ+ cells per 30.000 CD8+ restimulated mouse spleen cells. A) mice 1–3, B) mice 4–6, C) merged data.
Figure 3.
Cellular immune responses induced by the DNA vaccine candidates (IFNγ production was measured using a commercial ELISPOT assay).
Balb/C mice (n = 6 per group) were inoculated IM with (i) pORT ConcTat NG, (ii) pORT Gag 203 , (iii) pORT Env MD , (iv) pORT Env NG9 , (v) pORT gp120 CN54 (positive control), (vi) pORT1a clade (negative control). One week after the second/third booster immunization, spleen cells were isolated and tested for specific cellular immune responses by measuring IFN-g production after stimulation with pools of overlapping 15-mer peptides. Shown is the total number of positive (IFNγ secreting) cells per 106 restimulated mouse spleen cells. A) mice 1–3, B) mice 4–6, C) merged data.