Figure 1.
Developmental stages of the barnacle Balanus amphitrite that were sampled for transcriptomic and gene expression analysis.
The stages include stage II nauplius, stage VI nauplius, cyprid, juvenile and adult. The larval cDNA pool consisted of stage II nauplius, stage VI nauplius and cyprid, while the adult cDNA pool was staged only. The diameter of the adult barnacle is ∼2 cm. Scale bars = 100 µm.
Figure 2.
The size distributions of the contig/isotigs (A), the total ESTs (B), and the deduced open reading frames (ORFs) (C).
Table 1.
Summary of the barnacle B. amphitrite transcriptome.
Figure 3.
Top 30 gene ontology (GO) categories of B. amphitrite transcriptome.
Figure 4.
The MA-plot generated by the DEGseq gene expression profile analysis.
The M is the reads number ratio and the A is the average. The red dots indicate the differently expressed genes between the larval and adult cDNA pools.
Figure 5.
Phases of the larval settlement process in B. amphitrite.
Functional gene groups and genes that were differentially expressed and may be involved in this process are indicated in Table S2.
Figure 6.
Real-time PCR results for the expression of 18 genes in five different developmental stages.
The stages include the stage II nauplius (nau2), the stage VI nauplius (nau6), the cyprid (cyp), the young juvenile (juv) and the adult (adu). Expression was measured for (A) Ba-vtg, vitellogenin; (B) Ba-mnr1, mannose receptor 1; (C) Ba-mnr2, mannose receptor 2; (D) Ba-mnr3, mannose receptor 3; (E) Ba-mnr4, mannose receptor 4; (F) Ba-cph1, 20 kDa-cement protein homologue 1; (G) Ba-cph2, 20 kDa-cement protein homologue 2; (H) Ba-eph, epidermal growth factor receptor; (I) Ba-ptk7, tyrosine-protein kinase-like 7; (J) Ba-ddr1, discoidin domain receptor 1; (K) Ba-ddr2, discoidin domain receptor 2; (L) Ba-bursl, bursicon-like; (M) Ba-eir, ecdysone-inducible protein; (N) Ba-ecr1, ecdysone receptor 1; (O) Ba-ecr2, ecdysone receptor 2; (P) Ba-cubn1, cubilin 1; (Q) Ba-cubn2, cubilin 2; and (R) Ba-cubn3, cubilin 3. Values are expressed as mean ± SD from three different experimental replicates.
Figure 7.
Gene spatial expression patterns of B. amphitrite mannose receptor genes, Ba-mnr1 (A, B) and Ba-mnr2 (C, D).
(A) lateral view of a cyprid; (B) negative control; (C) lateral view of a cyprid; (D) negative control. Abbreviations: Ant, antennule; Cc, cyprid bivalve carapace; Ce, compound eye; Ta, thorax; Th, thoracopod. Scale bars = 100 µm.
Figure 8.
Gene spatial expression pattern of B. amphitrite 20-kDa cement protein homologue genes, Ba-cph1 (A, B, C) and Ba-cph2 (D, E, F).
(A) lateral view of a cyprid; (B) dorsal-lateral view of a cyprid; (C) negative control; (D) lateral view of a cyprid; (E) dorsal view of a cyprid; (F) negative control. Abbreviations: Ant, antennule; Ce, compound eye; Cg, cement gland; Th, thoracopod. Scale bars = 100 µm.