Gene expression profiling of committed mESCs in perfusion.
Relative expression of genes expressed in mESCs (Klf4, Rex1) and epiblast (Sox17, Dnmt3b, T, Fgf5) for cells in perfusion culture (N2B27+FGF4 at 5 ng/mL), normalized to GAPDH and to self-renewal static culture (N2B27+LIF+BMP4). Data are shown as average ± s.d. from 4 independent experiments, (* Indicates statistical significance, ° P = 0.06, * P<0.05, ** P<0.01, *** P<0.001 for logarithmic distribution). The large variability in FGF5 gene expression is typical for this gene [26].
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