Figure 1.
Syntheses of designed chemicals.
(A) Br2, AlCl3, ether; (B) 1,2,4-triazole, K2CO3, acetone; (C) R2X, 60% NaH, DMF, reflux; (D) NaBH4, MeOH.
Figure 2.
Effect of chemicals on 2-week-old rice seedlings.
(A) epi-5DS levels in root exudates of 1 µM chemical-treated rice seedlings determined by LC-MS/MS. The data are means ± SD of three samples. (B) The length of 2nd leaf sheath in 10 µM chemical-treated rice. The data are means ± SD of six samples.
Figure 3.
A comparison of the effects caused by TIS13 and TIS108 treatment.
(A) Chemical structures of strigolactone biosynthesis inhibitors. (B) epi-5DS levels in root exudates of chemical-treated rice seedlings determined by LC-MS/MS. The data are means ± SD of three samples. (C) The length of 2nd leaf sheath in 10 µM chemical-treated rice. The data are means ± SD of six samples.
Figure 4.
Effects of TIS108 on 2-week-old rice seedlings.
(A) epi-5DS levels in roots and root exudates of TIS108-treated rice seedlings determined by LC-MS/MS. (B) Germination stimulant levels in culture media of 1 µM TIS108-treated rice seedlings using Striga seeds. DW, distilled water; GR24, 1 µM GR24; control, non-treated rice culture media; TIS108, culture media of 1 µM TIS108-treated rice; TIS108+GR24, culture media coincubated with 1 µM GR24 and 1 µM TIS108. The data are means ± SD of three samples.