Table 1.
Characteristics of the study population.
Table 2.
Plasma cell free DNA (cf-DNA) values in patients with bacteremia stratified by various demographic features, underlying conditions and causative organism.
Figure 1.
A Line plot diagram showing cell free DNA (cf-DNA) concentration detected on days 1–4, 5 to 17 days and >25 days after blood culture in nonsurvivors (black plots) and in survivors (open plots).
Table 3.
Plasma cell free DNA concentration during days 1 to 4 after blood culture in relation to bacteremia outcome.
Figure 2.
Receiver operating characteristic (ROC) curve for maximal plasma cell free DNA (cf-DNA) concentration detected on days 1–4 after positive blood culture in relation to case fatality in bacteremia patients.
Figure 3.
Cumulative 30-d survival in bacteremia patients with maximum plasma cell free DNA (cf-DNA) >1.52 ug/ml compared to those with cf-DNA ≤1.52 ug/ml. The cut off value of 1.52 ug/ml was chosen using ROC curve. The survival curve was calculated using the Kaplan-Meier method, and survival differences between groups were compared by log-rank test.
Table 4.
Clinical disease severity of patients stratified by maximum plasma cell free DNA (cf-DNA) value (1 to 4 days after blood culture).
Figure 4.
Qualitative analysis of plasma cf-DNA in 8 bacteremia survivors (panel A) and 8 non-survivors (panel B) after NucleoSpin® Plasma XS kit extraction. Analyses were performed with Agilent's High Sensitivity Lab-on-a-chip DNA assay. Green lines indicate the low-weight (35 bp) DNA marker and purple lines the high- weight (10 380 bp) DNA marker. TP 1 = time point 1 (1 to 4 days after blood culture), TP 2 = time point 2 (5 to 17 days after blood culture), TP 3 = time point 3 (recovery, >25 days after blood culture). The intensity of the low-molecular-weight cf-DNA band was graded as follows: 1 = no visible cf-DNA or weak intensity, 2 = intermediate intensity, 3 = strong intensity. Abbreviations; bp = base pairs, TP = time point.
Table 5.
Spearman′s test correlation between cf-DNA level and C-reactive protein (CRP), soluble Fas (sFas), Fas ligand (FasL), kynurenine to tryptophan ratio (kyn/trp) and visually graded band intensity in gel electrophoresis.