Figure 1.
CD4+CD25highCD39+ T cells express increased levels of Treg markers.
FoxP3 (A), CTLA4 (B) and GITR (C) expression, as measured by flow cytometry, are increased in CD4+CD39+, as compared to CD4+CD39− T cells, especially in the CD25high compartment.
Figure 2.
Treg markers are up-regulated in RR-MS patients experiencing clinical relapses.
A–E. Clinically relapsing RR-MS patients displayed increased PBMC mRNA levels for CD25 (A), CTLA-4 (B), GITR (C), CD39 (D), and foxp3 (E). We also found significantly lower levels of Treg markers mRNA in stable RR-MS patients as compared to healthy controls (HC). Values are expressed as arbitrary units (AU). P values are indicated (Mann-Whitney).
Figure 3.
FoxP3+CD39+ Treg cells are increased during acute MS.
Scatter plots from three representative subjects, one healthy donor (HC; A), a stable MS patients (B), and a patient experiencing a clinical re-exacerbation of MS (C) are shown, indicating that the FoxP3/CD39 double positive T cell population (A, right panel) in the CD25high gate dramatically decreases during stable MS (B, right panel) and is restored during an acute attack (C, right panel).
Figure 4.
Treg cells are increased during acute MS.
The percentage of FoxP3+ cells in the CD4+CD25high gate is shown in (A) in healthy controls (HC; n = 14), stable MS (n = 10), and acute MS (n = 8). The percentage of CD39+ cells in the CD4+CD25high gate is shown in (B) in HC (n = 44), stable MS (n = 31), and acute MS (n = 32), and, similarly, the percentage of FoxP3+/CD39+ cells in the CD4+CD25high gate is displayed in (C) in HC (n = 13), stable MS (n = 12), and acute MS (n = 11). Again, while Treg cells, as defined by these markers, were significantly decreased in stable MS patients and restored during an acute attack. Lines represent median values, and P values are indicated where significant (Mann-Whitney). CD4+CD25highCD39+ regulatory T cells from an acute MS patient suppress T responder cell proliferation (E) in a dose dependent way (F), as measured by CFSE dilution assay. Plots represent CFSE-labeled T responder cell proliferation in absence and presence of regulatory T cell (5∶1) (E, F). A representative experiment among three is shown. Treatment-free RR-MS patients (n = 15) were followed longitudinally every two months for 14 months (G–H), and divided according to the occurrence of clinical relapses during the follow-up in stable (G), or relapsing (H) patients. PBMC foxp3 mRNA values, normalized on CD4 and GAPDH mRNA, and expressed as arbitrary units (AU) are plotted.