Figure 1.
Neighbor-joining phylogenetic tree of Candidate Division TM7 with 255 TM7 phylotypes, where 160 are identified as being environmental, 42 as animal-associated, and 53 as human-associated, with a total of 208 unique OTUs.
The bar chart above the outer ring represents the clone counts for each of the 16 phylotypes in our study. Subdivisions 1 and 2 are marked with a gray banner on the inner border of ring. Bootstrap values of major branches are indicated according to legend. An interactive version of this tree (http://www.phylotouch.com/tm7), developed with jsPhyloSVG [36], includes meta-analysis data such as distance matrices and links to sequences and publications.
Figure 2.
Quantification of Bacteria, TM7, and TM7a-like phylotypes by (A) qPCR and (B) FISH.
Average number of (A1 and B1) Bacteria cells mL−1, (A2 and B2) Candidate Division TM7 cells mL−1, and (A3 and B3) TM7a-like cells mL−1. Closed circles indicate undetermined quantification values. Error bars represent standard error of the mean.
Table 1.
16S rDNA gene amplification parameters for qPCR assay from activated wastewater.
Figure 3.
Micrographs depicting TM7 (red) and TM7a-like (blue) in the total microbial community (green) of activated sludge samples through (A–B) FISH and STARFISH (C1–C2).
(A) TM7a-like short filamentous cells with three segments labeled with both TM7 and TM7a probes. (B) TM7 and TM7a-like cells (latter marked with arrows) as coccobacilli and cocci; two insets show TM7a diplo-bacillus morphologies commonly found in sludge. (C) TM7 long filamentous cells (C1, red-orange) taking up a mixture of dissolved tritiated amino acids (C2, bright field) through micro-autoradiography (STARFISH) assay, suggesting TM7 are metabolic active in wastewater. Scale bars = 5 µm.