Figure 1.
Effect of neutrophil depletion on wound healing.
(A) Representative images of fluorescein-stained corneas at day 0 and day 2 after injury in control and neutrophil (N)-depleted WT and HO-2−/− mice. (B) Wound closure as percent change from day 0. Results are mean ± SE; n = 5–7; *p<0.005 from control WT mice; **p<0.005 from control HO-2−/− mice; † p<0.01 from control WT mice; ‡ p<0.005 from neutrophil-depleted WT mice.
Figure 2.
Infiltration of Inflammatory cells in the cornea at day 2 after epithelial injury.
(A) Representative H&E staining of corneas from uninjured and injured control and neutrophil-depleted WT and HO-2−/− mice 2 days after injury. (B) Number of neutrophils in peripheral blood of control and neutrophil (N)-depleted WT and HO-2−/− mice 2 days after injury. Results are mean ± SE; n = 3; *p<0.0001 from control WT; **p<0.0001 from control HO-2−/− mice; † p<0.05 from control WT.
Figure 3.
Immunofluoescence analysis of (A) Gr-1- and (B) CD68-positive cells in corneas from control and neutrophil-depleted WT and HO-2−/− mice 2 days after injury.
Images were taken at 63× magnification and are representative of three separate analyses.
Figure 4.
Real-time PCR analysis of HO-1 mRNA expression in neutrophils purified from peripheral blood of control and neutrophil (N)-depleted WT and HO-2−/− mice 2 days after injury.
Results are the mean ± SE; n = 3; *p<0.01 from neutrophils purified from control WT mice; **p<0.05 from neutrophils purified from neutrophil-depleted HO-2−/− mice.
Figure 5.
Neutrophil adhesion onto untreated (Control) and TNFα-treated WT and HO-2−/− aortic endothelial cells (mAEC).
Neutrophil adherence is measured as the fluorescence intensity associated with mAEC and is given in relative fluorescence units (RFU). Results are the mean ± SE; n = 3; **p<0.005 from WT mAEC; †p<0.05 from untreated HO-2−/− mAEC.
Figure 6.
Real-time PCR analysis of mRNA expression of (A) midkine (Mdk) and (B) VE-Cadherin in untreated aortic endothelial cells (mAEC) from WT and HO-2−/−.
Results are the mean ± SE; n = 4 to 5; *p<0.005 from WT mAEC.