Table 1.
Concentration-dependent mortality at 5 dpf after 96 h exposure.
Table 2.
Zebrafish embryo LC50 values found in this study, and the corresponding rodent LD50 values based on the literature.
Figure 1.
Cumulative mortality and infertility of zebrafish in buffer or egg water.
Embryos were kept in 92 mm Petri dishes with 40 ml of either buffer or egg water, 60 eggs per dish. Each error bar represents ±SEM of N = 420 embryos each for buffer and egg water. A, cumulative infertility and early mortality in buffer. B, the same, in egg water. There is no significant difference between the two media in terms of survival and fertilization percentage.
Figure 2.
Rate of evaporation from 96-well plates at 28.0°C.
Buffer was dispensed in four different 96-well plates. A, cumulative average percentage buffer loss per plate. All wells were initially filled with 250 µL buffer. B, percentage buffer loss after 96 h, per well, as a function of well position. The letters A–H and the numbers 1–12 correspond to the standard coordinates embossed into 96-well plates. All wells were initially filled with 250 µL buffer. Only the wells with grey columns were measured.
Figure 3.
Correlation between zebrafish embryo Log LC50 and rodent Log LD50 for the 60 compounds tested in this study.
Zebrafish embryo LC50 was determined based on cumulative mortality after 96 h exposure of compounds from three independent experiments and rodent LD50 was taken from the literature. Key: blue, regression line; solid black lines, 0.25 and 0.75 quartiles; dashed line, perfect correlation line. The slope of the regression line (blue) is 0.73403.
Figure 4.
Linear regression model: rodent log LD50 and zebrafish embryo log LC50.
The effect of the different compounds on the slope and intercept of the ANCOVA model. Although we must consider the effect of the unknown error in the rodent LD50 values, the different compound classes seem to cluster in different regions in the graph.
Table 3.
Statistical analysis of regression per group of compound using the ANCOVA model described in the text.