Figure 1.
Antioxidant capacity of mung bean soup.
The total antioxidant activity (T-AOC) of MBS and the bean cotyledon soup made from the same quantity of mung bean. Data represent mean ± s.d.; n = 3.
Figure 2.
Comparison on antioxidant capacity.
Comparing the MBS (in d.m. 20 mg /mL) with a tea soup (in d.m. 5 mg/ml) and a vitamin-C solution (0.15 mg/ml) on DPPH·-SA (A), O2·-SA (B) and OH·-SA (C). Data represent mean ± s.d.; n = 3.
Figure 3.
Contents of vitexin and isovitexin in mungbean.
Contents of vitexin and isovitexin in mungbean were quantitatively measured by the HPLC system. A model solution was prepared according to levels of vitexin (157.5 mg/100 g) and isovitexin (198.5 mg/100 g) in mungbean with the purified vitexin and isovitexin by a semi-prepared HPLC system. Data represent mean ± s.d.; n = 3.
Figure 4.
Attribution of vitexin and isovitexin in the antioxidant capacity of MBS.
Vitexin and isovitexin purified by a semi-prepared HPLC system were analyzed on T-AOC (A), DPPH•-SA (B), ABTS·+-SA (C). Data represent mean ± s.d.; n = 3.
Figure 5.
Bioavailability of vitexin and isovitexin in rat.
For analyzing in vivo absorption of the flavonoids from MBCE and its effect on T-AOC in plasma, the rats were fed with MBCE (1000 mg/kg BW) via gavage. The plasma samples were collected at the defined times and used for measuring the T-AOC (A) and quantitative analysis of vitexin and isovitexin by HPLC (B). Data represent mean ± s.d.; n = 6.
Figure 6.
Effects of pre-administration of MBCE against heat stress in rat.
For analyzing effects of MBCE against heat stress, the rats were fed with MBCE (100, 200 or 400 mg/kg BW) at 10 min before heat treatment and collected plasma samples immediately after the heat-treatment. T-AOC (A), MDA content (B), GSH content (C), LDH activity (D) and NOS activity (E) were determinate. Data represent mean ± s.d.; n = 6.
Figure 7.
Effects of post-administration of MBCE against heat stress in rat.
For analyzing effects of MBCE against heat stress, the rats were fed with MBCE (100, 200 or 400 mg/kg BW) immediately after the heat treatment and collected plasma samples at 40 min after gavage. T-AOC (A), MDA content (B), GSH content (C), LDH activity (D) and NOS activity (E) were determinate. Data represent mean ± s.d.; n = 6.