Figure 1.
Screening for the susceptibility of four different diatom species to K. algicidafiltrate.
Mean values of in vivo fluorescence + SD (n = 4) displayed are measured after 39 h. Asterisks indicate significant differences between the respective control and treatment.
Figure 2.
Effects of size fractionated S. costatum, K. algicida and co-culture cell free filtrates on the susceptible species S. costatum determined by in vivo fluorescence.
Values displayed are measured after 47 h and are mean values + SD (n = 5 for all but S. costatum where n = 4). Different letters indicate statistically significant differences.
Figure 3.
Effect of the protease inhibitor PMSF on the inhibiting effect of K. algicida filtrates.
Values displayed are measured after 52 h and are mean values + SD (n = 5).
Figure 4.
Left: Growth of S. costatum indicated as in vivo fluorescence in K. algicida filtrate conditioned for 24 h with S. costatum, C. didymus or seawater.
Right: Incubations with the same filtrates that were heat inactivated before the start of the experiment. Displayed are mean values + SD (n = 6) taken 70 h after the start of the experiment. A statistically significant difference is indicates by different letters above the bar.
Figure 5.
Protease release by K. algicida during the growth of a culture.
A) Protease release pattern of K. algicida under standard growth conditions. B) Protease release pattern of K. algicida with conditioned cell free filtrate added directly with the inoculation of the cultures. C) Protease release pattern of K. algicida with conditioned cell free filtrate added 16 h after the inoculation of the cultures. The line indicates bacterial growth measured as OD and the bars give the bacterial protease release rate. The arrows indicate the time of the addition of K. algicida conditioned cell free filtrate. Displayed are mean values + SD (n = 3).