Figure 1.
Overview of data analysis steps.
This diagram shows the approach that was undertaken in this study in order to use gene expression data for fine mapping a behavioral QTL.
Figure 2.
LOD scores of behavioral and expression QTLs.
Plots of LOD scores across chromosome 15 (where markers available) are given for DM1 (distance moved on day 1) and FVSOP1 (frequency of visits to exposed platform on day 1), generated using a chromosome substitution strain F2-population. Open circles indicate the –LOD 1 support interval. In addition, the expression QTL is plotted for Ly6a, the only gene appearing in the results of both expression analyses. The black arrow indicates its location (74,825,308 bp).
Table 1.
Genes on chromosome 15 resulting from eQTL analysis.
Figure 3.
Network construction identifies distinct modules of co-expressed genes using hippocampus samples of CSS15F2 population (n = 37).
The dendrogram was produced by average linkage hierarchical clustering of genes using topological overlap. Modules of co-expressed genes were assigned colors corresponding to the branches indicated by the horizontal bar beneath the dendrogram.
Figure 4.
Behavioral analysis of Ly6a−/− mice confirms involvement in novelty induced phenotypes.
Ly6a−/− (n = 8) and wildtype animals (n = 9) were tested in an automated open field system for 30 minutes. The data was analyzed using a repeated measures ANOVA for 6 bins of 5 minutes, after which individual bins were tested using independent t-tests (Bonferroni corrected). Total distance moved (in cm) is depicted in the bar diagram. The line graph shows the distance moved of Ly6a−/− and wildtype mice over the six bins. The error bars represent the standard deviation (SD). Stars indicate significance (Bonferroni corrected p<0.05).