Figure 1.
The Rosa hybrida L. cultivars used as parents of the segregating population (GGFC).
A. Rosa hybrida cv. Golden Gate (GG) is a modern cut-flower cultivar, containing carotenoids that are responsible for its yellow color, with only faint odor and long vase life. B. Rosa hybrida cv. Fragrant Cloud (FC) is an old garden cultivar with large fragrant flowers, short vase life and peach red petals color due to the presence of anthocyanins (Short movie presenting the vase life behavior of these cultivars is available at http://www.youtube.com/watch?v=odOp92TK5Xg - the movie composed of pictures that were taken every 60 minutes with total time lapse of 15 days).
Table 1.
The markers used to construct the rose map.
Figure 2.
Each map consists of seven linkage groups. Map distances are shown in cM as a ruler at the left page margin. Marker names are assigned according to the nomenclature described in Table S1. Each color represents a different segregation ratio (black for 1∶1, red for 5∶1, blue for 3∶1 and green for codominant markers). A. Map of Fragrant Cloud (FC). B. Map of Golden Gate (GG).
Table 2.
Distribution of markers on parental maps (GG and FC) and linkage group statistics.
Figure 3.
The linear order of common markers conserved in both parental maps.
Each linkage group name contains the parental name and the linkage group number. Map distances are shown in cM as a ruler at the left page margin. Marker names indicated according to the nomenclature described in Table S1. Each color represents a different segregation ratio (black for 1∶1, red for 5∶1, blue for 3∶1 and green for codominant markers). Common markers are indicated in bold and larger font. A. Linkage group 5. B. Linkage group 7. The remaining linkage groups are presented in Fig. S5.
Figure 4.
A comparison of the tetraploid GGFC maps with the diploid ICM.
A. Number of markers of each diploid ICM linkage group that correspond to the autotetraploid linkage groups of the GGFC maps. Each marker is indicated by a black dot. Cells that contain more than one marker are noted with a grey background. B. The linear order of conserved markers between the FC map and ICM maps. Results are shown for FC2-ICM2 and FC4-ICM7. Map distances are shown in cM as a ruler at the left page margin. The linkage group numbers appear above each group. FC marker names are indicated according to the nomenclature described in Table S1. Each color represents a different segregation ratio (black for 1∶1, red for 5∶1, blue for 3∶1 and green for codominant markers). The ICM marker names and positions are given according to Spiller et al [27]. Black lines connecting the common markers. The markers that present on the ICM but not connected to the FC map are corresponding to the GG map.
Figure 5.
Rosa FC-Fragaria pseudo-chromosome comparison.
The number of markers of each autotetraploid FC linkage group (RG) that correspond to the pseudo-chromosomes of the Fragaria FvH4 reference sequence (FvChr). Each marker is indicated by a black dot. Cells that contain more than three markers are shaded with a grey background. The tentative relationship between RG6 and FvChr4 (two markers) is shown with a dark grey background.
Figure 6.
Comparison between the Rosa FC linkage map and the pseudo-chromosomes of Fragaria FvH4 genome sequence.
Each group contains the map positions of the 70 orthologous markers used for comparison. Lines between Rosa linkage groups (RG) and Fragaria pseudo-chromosomes (FvChr) indicate marker positions within syntenic blocks. Map distances are given in cM, pseudo-chromosome positions are given in nucleotides. Marker names are given with the suffix according to the Rosa linkage group on which they are mapped. Markers common to syntenic blocks are given in the color of the Rosa linkage group; non-syntenic markers are given in grey. Delimiters defining the ends of the Fragaria pseudo-chromosomes and where necessary the Rosa linkage groups are given in grey with the pseudo-chromosome/linkage group name.
Figure 7.
Two of the Fragrant Cloud (FC) linkage groups showing the four homologous chromosomes (1–4).
Map distances are shown in cM as a ruler at the left page margin. Marker names indicated according to the nomenclature described in Table S1. Each color represents a different segregation ratio (black for 1∶1, red for 5∶1, blue for 3∶1 and green for codominant markers). A. Linkage group 2. B. Linkage group 5.