Figure 1.
MCF10A cell motility atop 2D matrices.
(A) Ductal/single lobe cross-sectional depiction of MCF10A breast cancer progression series: 10A.vec (non-transformed), 10A.ErbB2 (partially transformed), 10A.14-3-3ζ (partially transformed), and 10A.ErbB2.ζ (fully transformed). (B) Mean cell migration speed <S> atop 2D matrices; p-values are with respect to <S> of 10A.vec cells. (C) Cell population persistence time P atop 2D matrices (average R = 0.87). All p-values (*,p≤0.05; **, p≤0.01; ***, p≤0.001) determined from t-tests for unpaired samples.
Figure 2.
MCF10A cell motility in 3D matrices.
(A) Scanning electron micrographs of compliant (104 Pa) and stiff (391 Pa) 3D matrices; scale bar is 2 µm. (B) Mean cell migration speed <S> in compliant 3D matrices. (Inset) cell sphericity Ψ as taken from Baker et al. [17]; p-values are with respect to <S> (and Ψ) of 10A.vec cells. (C) Mean cell migration speed <S> in stiff 3D matrices; p-values are with respect to <S> of 10A.vec cells. (D) Percent decrease in <S> of cells within compliant matrices relative to cells in stiff matrices. The p-values shown in black reflect the difference in <S> between cells within compliant matrices and the same cells within stiff matrices; the p-values shown in red reflect the difference in % decrease in <S> among the sublines. (E) Cell population persistence time P in compliant and stiff 3D matrices (average R = 0.87). (F) Percent decrease in <S> of cells atop 2D matrices relative to cells within compliant 3D matrices. The p-values shown in black reflect the difference in <S> between cells atop 2D matrices and the same cells within compliant 3D matrices; the p-values shown in red reflect the difference in % decrease in <S> among the sublines. All p-values (*,p≤0.05; **, p≤0.01; ***, p≤0.001) determined from t-tests for unpaired samples.
Figure 3.
Windrose plots of MCF10A cell migration.
Top row lists cell line; left column lists matrix condition. Cells in 2D matrices exhibited the highest degree of motility, followed by cells within compliant (104 Pa) 3D matrices and then by cells within stiff (391 Pa) 3D matrices.
Figure 4.
Single-plane confocal images of MCF10A cells embedded within 3D Type I collagen matrices and cultured atop 2D Type I collagen matrices.
Top row lists cell line; left column lists matrix condition. Yellow carats indicate thin, rod-like cellular processes. Green arrows indicate tubular-shaped cellular protrusions. Blue brackets indicate sheet-like cellular protrusions.