Table 1.
PCR primers and amplicon sizes for prophage screening.
Table 2.
Serotypes of S. agalactiae isolates from blood cultures and CSF from adults and neonates.
Figure 1.
Genetic diversity and sequence type (ST) distribution, determined by MLST [10], of 142 S. agalactiae isolates from cases of adult (AI) and neonatal (NI) invasive disease.
We show the phylogenetic network applied to 43 parsimonious-informative sites from a total of 3,456 nucleotides generated with the neighbour-net algorithm for the 142 strains studied (http://splitstree.org/) [29]. Strains were grouped into clonal complexes (CCs) with eBURST software (http://eburst.mlst.net/). Columns indicate the percentages of AI and NI strains in each CC. Recombination (R) and mutation (M) rates, based on MLST data, were evaluated as described by Feil et al. [31]. The estimated recombination-mutation ratio (R/M) varied as a function of the CC to which the strain belonged.
Table 3.
Serotype of S. agalactiae isolates from the various STs and CCs implicated in adult and neonatal invasive infections.
Table 4.
CC, ST of S. agalactiae isolates from adult and neonatal invasive infections.
Table 5.
Distribution of the SLVs of the major clonal complexes as a function of difference in the number of nucleotides (one or more) with respect to the sequence of the founder sequence type.
Figure 2.
Distribution of 141 S. agalactiae isolates from adult (ACSF and AB) and neonatal (NCFS and NB) patients with invasive disease between prophage DNA groups, on the basis of PCR evaluations of the prophage content of isolates.
Jaccard analysis generated a dendrogram of similarity values for the 10 prophage sequences described in table 1 (SYSTAT 12 software). Five major prophage DNA groups were defined (groups A to E). The mean number of prophage DNA fragments amplified from strains by PCR and the mean number of absolute deviations (Avedev) were calculated for each prophage DNA group. a anatomic origin of isolates; b serotype of isolates; ST, sequence-type; CC, clonal complex; NT, nontypeable.
Table 6.
Distribution of the S. agalactiae isolates of various origins, serotypes, sequence types, and clonal complexes between prophage DNA groups, as displayed by SYSTAT 12 softwarea.