Table 1.
Distribution of ABH and Lewis Blood groups in the studied individuals.
Table 2.
Comparison of FUT2 genotypedeterminations with secretor phenotype determinations.
Figure 1.
Richness and diversity of dominant bacteria in faecal samples of the non-secretor and secretor individuals (A, B) and of the Lewis a, b and negative individuals (C, D) based on the DGGE profiles.
A significant difference between the groups by ANOVA: * p<0.05. In addition, a trend (p = 0.07) towards higher diversity in the non-secretor than in secretor individuals and towards higher richness in Le b individuals than in Le negative individuals was detected.
Table 3.
The significantly differing band positions and the incidence of bands in secretor (14) and non-secretor samples (57) by PCR-DGGE with universal bacterial primers.
Figure 2.
PCA plot based on the bifidobacterial DGGE profiles of faecal samples from the non-secretor (open circles) and secretor (closed circles) individuals (A) and DGGE bands contributing to the principal components 1 and 2 (B).
In panel B, the numbers in bold indicate the band positions, which were significantly less commonly (Fisher's exact test, p<0.01) detected in the non-secretor individuals than in the secretor individuals (See Table 4).
Table 4.
Identification of the bifidobacterial DGGE band positions by sequencing and the incidence of the bands in secretor (14) and non-secretor samples (57).
Figure 3.
Bifidobacterial richness and diversity in faecal samples of the non-secretor and secretor individuals (A, B) and Lewis a, b and negative individuals (C, D) based on the DGGE profiles.
Significant differences by ANOVA: **** p<0.0001, *** p<0.001.
Figure 4.
Incidence (% of samples) (left) and Box-and-Whisker plots (right) (based on log10 16S rRNA gene copies per g faeces) of total bacteria, bifidobacteria and bifidobacterial groups in the faecal samples of the non-secretor and secretor individuals by qPCR.
A significant difference by Wilcoxon test: * p<0.05. In addition, a trend (p = 0.06) towards higher number of the 16S rRNA gene copies of B. adolescentis in the secretor individuals than in the non-secretor individuals was detected.
Table 5.
Primers used in sequencing of the FUT2 gene encoding fucosyltransferase 2.
Table 6.
Primers targeting the 16S rRNA gene, annealing temperature and strains used as standards in qPCR analysis.