Figure 1.
Migration and phenotype of DC in lung during Pb infection.
A- At several time points after Pb infection, the absolute numbers of DC (MHC-II+/CD11c+) were determined in the lung. Error bars represent the SEM. B-Phenotype of lung DC after Pb infection, the gate represent positive cells, determined by FMO, as described in the M&M. This figure is representative of 3 different experiments. The numbers represent the percentage of positive cells. The experiment was performed at least 3 times. *p<0.05 when compared with PBS treatment.
Figure 2.
Migration of BM-DC to the lymph nodes.
BM-DC were incubated with Pb labeling with CFSE and injected into the lung. After 12 and 24 hrs the lymph nodes cells were analyzed by citometry. A-Figure representative of 3 different experiments, where the gate represent positive cells, determined by FMO, as described in the M&M. The numbers represent the percentage of positive cells. B- At 12 and 24 hrs after Pb infection, the absolute numbers of CFSE+/CD11c+ were determined in the lymph nodes. Error bars represent the SEM. *p<0.05 when compared with only DC.
Figure 3.
Migration of lung DC to the lympho nodes.
CFSE and Pb were injected into the lung and after 12 and 24 hrs the reginal lympho nodes cells were analyzed to the presence of CFSE+/CD11c+ by citometry. A-Figure representative of 3 different experiments where the gate represent positive cells, determined by FMO, as described in the M&M. The numbers represent the percentage of positive cells. B- At 12 and 24 hrs after CFSE and Pb infection, the absolute numbers of CFSE+/CD11c+ were determined in the lympho nodes. Error bars represent the SEM. *p<0.05 when compared with only CFSE.
Figure 4.
DC transport yeast form of Pb to draining lymph nodes.
Yeast form of Pb were labeling with FITC as described in the M&M. After labeling, 1×106 Pb were injected intratracheally. After 12 h, the lymph node cells were removed and analyzed by cytometry. Animals injected with FITC only were used as controls. The Figure is representative of 3 different experiments. The number represent the percentage of positive cells.
Figure 5.
Lymph node T-CD4 cell activation by DC.
Animals were injected with Pb or Pb-pulsed BM-DC, and intracellular cytokines from T cells present in the lymph nodes were investigated. The data represent the mean ± SEM of the results from 3 independent experiments. Error bars represent the standard errors of the means (SEM). *p<0.05 when compared between DC vs. DC+Pb infection or #p<0.05 when compared between PBS vs. Pb infection.