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Table 1.

Ethogram used for the assessment of maternal care behavior in C57BL/6N and Balb/c dams.

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Figure 1.

Effects of strain and genotype on maternal care behavior.

Behavioral strain differences between C57BL/6N and Balb/c mothers with a glucocorticoid receptor wildtype (GR +/+) or a heterozygous deletion (GR +/−) are exemplarily presented for four different behavioral measures: (A) ‘licking/grooming’, (B) ‘passive nursing’, (C) ‘self-grooming out of nest’ and (D) ‘climbing/digging’. While strains were found to differ significantly in all four measures, GR genotype did not affect the behavior. Moreover, a significant strain-by-genotype-interaction was found with respect to ‘licking/grooming’. While C57BL/6N +/+ dams spent more time ‘licking/grooming’ than Balb/c mothers of both GR genotypes, no difference was found between C57BL/6N +/− mothers and Balb/c dams. Data are presented as untransformed means ± standard error of the mean, * p<0.05, ** p<0.01.

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Table 2.

Summary of all data generated by behavioral observations and the pup retrieval test.

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Figure 2.

Effects of strain and genotype on maternal behavior in the pup retrieval test.

Behavioral strain differences between C57BL/6N and Balb/c mothers with a glucocorticoid receptor wildtype (GR +/+) or a heterozygous deletion (GR +/−) are presented for two out of three behavioral measures: (A) ‘handling’ and (B) ‘crouching over pups’. Strains differed significantly in ‘handling’, but GR genotype did not affect any of the behavioral measures. However, a tendency for a strain-by-genotype-interaction was found with respect to ‘crouching’. Data are presented as untransformed means ± standard error of the mean, T p<0.1, * p<0.05.

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