Figure 1.
Phylogenetic tree of bacteria affiliated to the genus Pseudoalteromonas based on 16S rRNA gene sequences (5′-prime region, poisitions 10 to 509 E. coli equivalent).
Nucleotide distances are based on the maximum likelihood algorithm and the tree clustered using the Neighborjoining procedure.
Figure 2.
Percentage of larvae undergoing metamorphosis in response to biofilms of Pseudoalteromonas strain J010 at a range of bacterial densities.
Each data point represents the mean percentage of metamorphosis (±SE) of 6 replicates containing 10 larvae each, and the mean bacterial density (±SE) of 6 replicates with 10 cell counts in each replicate.
Figure 3.
Isolation of the bacterial metabolite of Pseudoalteromonas strains J010 that induced metamorphosis of coral larvae.
The asterisk marks 100% larval metamorphosis in bioassays after 6 h.
Figure 4.
HPLC chromatogram of the inductive C18-chromatographic fraction of Pseudoalteromonas strain J010.
The asterisk marks the peak fraction that induced 100% metamorphosis of coral larvae.
Figure 5.
Different types of larval response (settlement, metamorphosis, swimming) to a 10-fold dilution series of synthetic tetrabromopyrrole in absence (A, B) and presence (C, D) of chips of Hydrolithon onkodes after 6 h (A, C) and 24 h (B, D).
The control contained FSW only. The 1x concentration was lethal to the larvae. Each value (error bars) represents the mean (±SE) of 6 replicates with 10 larvae in each replicate.