Table 1.
Kinetic constants of the LD5 and SpA binding to three types of human Igs1.
Figure 1.
The enzyme-labeled LD5 exhibits enhanced binding activities for IgM and IgA.
The binding activities of horseradish peroxidase (HRP)-labeled LD5 (HRP-LD5) or HRP-conjugated goat anti-human polyclonal antibodies (HRP-goat anti-human PcAb) to coated hIgM (A), hIgG (B) or hIgA (C) were examined by ELISA. The coating buffer was used as solvent control.
Table 2.
Comparison of anti-HCV detection against the national reference panel of HRP-LD5 assay with three commercial diagnostic kits.
Figure 2.
Anti-HCV detection in 195 serum specimens from hemodialysis patients by different ELISA assays.
, A450 values, with ELISA cut-off values of either 0.150 indicated in solid line (established anti-HCV IgG assay and Chang Zheng anti-HCV diagnostic ELISA kit) or 0.200 indicated in dotted line (established anti-HCV IgM assay and HRP-LD5 based assay).
Table 3.
Comparison of anti-HCV antibody detection in serum samples from 195 hemodialysis patients of HRP-LD5 based assay with Chang Zheng anti-HCV kit.
Table 4.
IgM confirmation detection of five cases of HRP-LD5 assay positive alone serum samples*.