Figure 1.
A schematic map of the experimental design.
Figure 2.
Marker position and the recombination rate between neighboring markers.
(A) A schematic map of the 93 markers on 16 yeast chromosomes. The black triangle shows the position of centromere. The capital letter above the line is marker name, and the number under the line is the percentage of gametes being of Sc origin for the corresponding marker. Note that two markers 10A and 10B are of Sc origin in >90% of gametes, an observation that can not be reproduced in other Sc−Sp hybrids. (B) The recombination rate between neighboring markers is generally low. Three blocks (AB, BC, and CD) at the chromosome 10 were not included.
Figure 3.
No between-chromosome two-locus incompatibility was detected.
The fraction of heterogeneous combination ((ScSp+SpSc)/(ScSc+ScSp+SpSc+SpSp)) is shown for each of the 5111 block pairs. None is significantly different from 0.5, the expected value, at an FDR of 1%, as determined by Chi-square test. Note that the 4 blocks at the left arm of chromosome 10 were excluded from the analysis.
Figure 4.
The growth-based relative fitness is significantly (P<10−5; Z-test) higher than the sporulation-based relative fitness in all 89 F2 hybrids after Bonferroni correction for multiple testing.
The diploid Sp-YCM361 was used as the wild-type control in calculating relative fitness. Arrows point to strains that are not aneuploid. Error bar shows one standard error of the mean.
Figure 5.
Meiosis-related genes tend to be fast-evolving compared to other genes in Sc (P<10−3, Mann-Whitney U test).
The number of non-synonymous substitutions per non-synonymous site (dN) was calculated using Sp as the reference [34]. The proportion (y-axis) of genes with dN smaller than a certain number (x-axis) is shown.