Table 1.
Catfish species used in this study.
Table 2.
Summary of identification based on each species consensus barcoded sequence using BOLD Identification System (BOLD-IDS) and BLASTN search from GenBank.
Table 3.
Description of analyzed local market samples.
Table 4.
Estimates of Pairwise Genetic Distances between Catfish Species under Kimura 2-Parameter Model [18].
Figure 1.
Phylogenetic consensus tree of nine catfish species (and an Ictalurid hybrid) constructed using Maximum Parsimony (MP) Method.
The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) is shown next to the branches [21]. Hemibagrus macropterus was used as an outgroup. The MP tree was obtained using the Close-Neighbor-Interchange algorithm [20] with search level 1 in which the initial trees were obtained with the random addition of sequences (10 replicates). The source for each image was displayed next to the pictures.
Figure 2.
Multiple sequence alignment of consensus sequences for Ictalurus punctatus against voucher and reference sequences from GenBank and BOLD databases.
A multiple sequence alignment of Ictalurus punctatus was generated by ClustalW and graphically represented by BOXSHADE 3.21. The nomenclature of the aligned sequences is as follows: FISH_ID_Ictalurus_punctatus (as I. punctatus consensus sequence in the present study), GENBANK_Ictalurus_punctatus (as GenBank voucher species consensus sequence) and BOLD_Ictalurus_punctatus (as BOLD species reference consensus sequence). Both voucher and reference sequences were downloaded from the two databases with the accession numbers listed in Table S2. Highly conserved regions which were ≥50% identical were boxed in solid black and light shading indicates conservative substitutions.