Figure 1.
The strategy for target gene deletion in clostridial genome.
Figure 2.
Schematic representation of the construction of pMTL007-H1 for deletion of CAC1493-1494.
Figure 3.
The procedure for deletion of CAC1493-1494 (A) and ctfAB (B) in the genome of C. acetobutylicum.
Figure 4.
Construction of CAC1493-1494 deletion mutants.
A. Identification of an insertion mutant by PCR using primers Cac1494B and Pex1494E flanking the target site; B. Identification of the deletion mutants by PCR using primers P1492-5s and Pex1494E; C. Schematic show of operon CAC1493-1494 and the expected deleted operon CAC1493-1494 in the chromosome; D. Southern blot analysis of CAC1493-1494 deletion using CAC34 probe.
Table 1.
Bacterial strains, plasmids and primers.
Figure 5.
Construction of ctfAB deletion mutants.
A. Identification of an insertion mutant by PCR using primers Ct-5 and CTFB-3 flanking the target site; B. Identification of the deletion mutant by PCR using primers Pro2-5 and Pr2-3.