Table 1.
Methylation at Line-1 and F8 and its correlation with gender and age.
Table 2.
The influence of different hormone levels as fixed and women and month of cycle as random effects on different methylation measurements calculated by a mixed linear model is shown in this table.
Figure 1.
Average methylation levels at LINE-1 (SN9 and SN8) and Alus (SN1 and SN4) A) on three dates during the menstrual cycle (Date 1: during the menstruation phase, 55 samples; Date 2: near the ovulation phase, 56 samples; Date 3: during middle of secretory phase, 53 samples).
The data are the averages of all participating women and all measurements. B) of all blood samples taken from a given female donor. The graph shows the inter-individual variability between donors that is persistent over 3–4 months period. The donor number is given on the horizontal axis.
Table 3.
The stability of the methylation measurement at Line-1 and Alu both within (estimated by mean square of the residuals: Var_Error) and between women (estimated by mean square of the model: Var_Model) was calculated using the ANOVA approach.
Figure 2.
RT-PCR of the receptors in MDA-Kb2, T47-Kbluc, HEK293T, HUVEC cells and male and female peripheral blood derived cells.
Estrogen Receptor 1 (ER-1 (alpha)), Estrogen Receptor 2 (ER-2 (beta)), Progesteron Receptor (PR), and Androgen receptor (AR).
Figure 3.
Effect of Hormone treatment on DNA methylation levels at A) LINE-1 and B) Alu.
The 48 hours hormone response in HUVEC, T47D-KBluc and MDA cells. T and E stands for dihydrotestosterone and estradiol respectively. Each value corresponds to three different cell culture treatments, each measured thrice for methylation (making a total of 9 measurements).