Table 1.
Major strain distribution pattern for HS mice.
Table 2.
SNP genotyping in HS mice.
Figure 1.
Rarb mRNA expression in mouse brain.
Quantification of Rarb mRNA expression in whole brain by real-time RT-PCR. cDNA was prepared from the whole brains of uninfected 6–8 week old male mice or mice at the terminal stages of prion disease (Chandler/RML inoculated). N = 6 for all groups and samples were run in triplicate. All samples were duplexed for Rarb (Fam-label) and an endogenous control GAPDH, β-actin or Thy-1 (Vic-label). Expression level is expressed in arbitrary units as normalised by the geometric mean of the quantity of the endogenous controls (y-axis). Error bars represent the standard error of the mean. A. Rarb mRNA expression level for parental strain of the HS mice (except LP). B. Rarb mRNA expression level grouped by allele at SNP RARBX3 R107R (A/C) (A = A, AKR, BALB, C57; C = C3H, CBA, DBA). No significant difference was observed between the groups (P = 0.87) C. Comparison of Rarb mRNA levels in uninfected (dark bars) and mice at the terminal stage of disease (light bars). Significant differences are seen between normal and terminally sick mice (P = 3.0×10−6 and P = 3.1×10−5 for C57BL/6 and RIIIS/J respectively).
Figure 2.
Stmn2 mRNA expression in mouse brain.
Comparison of Stmn2 brain mRNA expression between uninfected and prion-infected mice at the terminal stage of disease (Chandler/RML inoculated) by real-time RT-PCR. N = 6 for all groups and samples were run in triplicate. All samples were duplexed for Rarb (Fam-label) and an endogenous control GAPDH, β-actin or Thy-1 (Vic-label). Expression level is expressed in arbitrary units as normalised by the geometric mean of the quantity of the endogenous controls (y-axis). Error bars represent the standard error of the mean. Dark grey and white bars represent uninfected and infected mice respectively. A significant difference is seen between normal and terminally sick mice for C57BL/6J (P = 2.0×10−6) but not for RIIIS/J (P = 0.08).