Figure 1.
Normalized QPCR evaluation of the common 4977-bp mtDNA deletion in TM of patients with different glaucoma types.
The horizontal axis shows the number of QPCR cycles, the vertical axis the amount of amplified DNA as inferred from the intensity of the fluorescence signal. The continuous curve indicates the normalized quantity of total mtDNA, the dashed curve indicates the relative amount of 4977 bp–deleted mtDNA. Samples in which the dashed curve intercepts the threshold horizontal line at lower QPCR cycles (left side) have a greater amount of 4977 bp–deleted mtDNA. For each sample, the box on the left shows oxidative nuclear DNA damage (8-oxo-dG) as detected by 32P-postlabeling.
Table 1.
Mitochondrial DNA damage detected in TM of various glaucoma types.